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Impact of measurable residual disease by decentralized flow cytometry: a PETHEMA real-world study in 1076 patients with acute myeloid leukemia
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Characterization of freshly isolated mesenchymal stromal cells from healthy and multiple myeloma bone marrow: transcriptional modulation of the microenvironment
The GTA Online Heist payout for completing the Cayo Perico job depends on a number of factors, but mainly on how many secondary target you secure along the way. During your recon you'll identify cash, weed, copyright, gold, and paintings that can be sold to the buyer in addition to the primary target, but as there's only so much that each player can carry, teaming up with others can help you score a larger payout overall.
Dr. Paiva: Absolutely. Originally, MRD testing was performed mostly with three different techniques, multiparameter flow cytometry that looks into live plasma cells and discriminates between the normal plasma cells which all of us have in our bone marrow as well as in secondary lymphoid tissues, peripheral blood, versus in myeloma plasma cells, which have a different phenotype. The way we discriminate between normal versus myeloma cells is according to the presence of the different phenotypes, aberrant antigen expression in the surface, as well as inside the myeloma plasma cell. Alternatively, there's also molecular PCR that is capable of detecting the DNA of patient's bone marrow samples. It's capable of detecting the clonotypic sequence of the monoclonal immunoglobulin that characterizes all myeloma plasma cells from every single patient. It's like the passport of the myeloma plasma cell. Now, both techniques have been there for myeloma, as well as for other hematological malignancies for now more than 10 or perhaps 15 years, PCR in particular, the most specific PCR approach, which is called ASO PCR. Allele-Specific Oligonucleotide PCR has perhaps been more sensitive than multiparameter flow, but is a handicap, the drawback of applicability. Because myeloma is an end-stage B cell disorder, it's also characterized by a higher rate of somatic hypermutations and this clearly limits the applicability of ASO PCR to monitor MRD in most myelomas. My contrast multi-parameter flow from 10 or 15 years is much more applicable to virtually all the patients, but was less sensitive. Nevertheless, all the available data -- and we now have more and more data and consistent data -- using both low sensitive flow and ASO PCR showed that both strategies as well as clear prognostic value even when performed among patients in complete response. In complete response, theoretically you don't have the disease anymore, but by using more sensitive measures, residual disease can still be identified.
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It’s possible that multiple Cayo Perico secondary targets appear at the same time and here are their values based on Hard mode Solo runs:
Jenny:Â I was just saying how significant I think it is that for the first time, you are studying the flow cytometry test to study the biology.
We also believe that -- and this into some extent might even be rational -- for example, now they've been back into the newly diagnosed setting. It is common knowledge that for example, gene expression profile signatures are of great value to discriminate between standard versus high-risk patients, but they are different from scientific group to scientific group. Our personal belief in this regard is that those signatures are different here because they were obtained with different treatment schemas. They worked in different trials, but they are obtained with different treatment schemas, which means that most likely, this strategy of trying to overcome the chemo resistance of MRD cells will be specific to the common backbone drugs beings used for each particular clinical trial because certainly, the nature of ultra chemo resistant cells will slightly differ according to the different drugs beings used.
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Jenny: Absolutely. Well, we agree and we're so thankful for the work you're doing, the study that's been done by you and your group. We're just very grateful because it's helping discover many new approaches and targets for myeloma.
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But when I'm invite my friend or other player to join my heist, it's get some weird problem and it happenned all the time.